Sylvester Comprehensive Cancer Center

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Flow Cytometry Core Facility

The Flow Cytometry Core Facility at Sylvester provides researchers with sophisticated methods for the analysis and preparative sorting of both normal and tumor cells. The facility helps researchers measure apoptosis, gene expression, drug metabolism, immune responses, and pathways of cellular activation, both normal and tumor-related.

The core maintains instruments, computer work-stations for data analysis, and related equipment to support scientists affiliated with Sylvester’s multidisciplinary research programs, as well as researchers in a wide variety of cancer-related research projects.

The facility also provides training to investigators in flow cytometric applications and cell sorting technologies.

In Development

In order continually to improve the Flow Cytometry Core Facility and the services it offers, the following areas are under consideration and/or development:

  • Modernization of the technology, and replacement of aging, unreliable instruments. For instance, the recent Bankhead-Coley shared instrument grant-funded installation of a custom-built LSR-Fortessa-HTS five laser analytical flow cytometer. The antiquated LSR-I analyser has now been replaced with a new, three laser FACS Canto-II instrument. The facility has also recently inherited a two laser Partec CyFlow Space analyser.
  • Online and software enhancements, including our new website-based scheduling system and FlowJo data analysis software.
  • Using our additional trained personnel, to provide on-demand extending hours of operation, with staggered shifts.
  • The satellite facility in the new Stem Cell Institute now is in operation, with a MoFlo XDP sorter. A second satellite facility, on the RMSB 8th floor, now houses an additional shared SORP Aria-II cell sorter.
  • Provision of a dedicated central computer server for off-line storage and archiving of data.
  • Promoting greater use of the available technology, such as multiple fluorochromes, BSL-2 sorting, flow cloning, etc.
  • Keeping abreast of new technology in the area of fluorescent dyes and other applications, by sponsoring appropriate presentations in flow cytometry by eg. BD, BCI and other leaders in the field.
  • Continually informing researchers of any new information, protocols and applications, by regular e-mail updates, as well as posting such information on this section of the Sylvester website and with announcements in the on-line instrument reservation system.
  • Providing further training in flow cytometer use and data analysis, to promote independent use of the core’s instruments, as well as to facilitate better user analysis of flow cytometry data.

Contact us

Phone

305-243-5571

Mailing Address

Rosenstiel Medical Sciences Building
1600 N.W. 10 Avenue
Room 3061, Locator R-138
Miami, FL 33136

Physical Address

Rosenstiel Medical Sciences Building
1600 N.W. 10 Avenue
Room 3061
Miami, FL 33136

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