Sylvester Comprehensive Cancer Center

Flow Cytometry Staff

To inquire about services of the Flow Cytometry Core Facility, contact Huw S. (“Zip”) Kruger Gray, Ph.D., Core Facility Director, at 305-243-5019 or HKGray@med.miami.edu. You can also contact any of the staff in the main laboratory facility (in RMSB 3061) at 305-243-5571.

Meet the Team

Huw S. (“Zip”) Kruger Gray, Ph.D., Core Facility Director

Dr. Kruger Gray oversees the operation of this core facility. His primary responsibilities include determining facility policies, providing consultation to researchers on experimental design, assisting with data interpretation, budgeting, determining future development strategy for the facility and seeking appropriate funding. Dr. Kruger Gray has over 20 years of experience in flow cytometry, including applications and technical support, both academic and industrial. He holds a Ph.D. in micro-biology from the University of Essex in England.

The experienced and talented facility staff jointly are responsible for all aspects of the daily operation of all flow cytometry instruments. They assist with and provide advice on running samples with users on the analyzers, as well as exclusively performing cell sorting. They also are responsible for performing regular quality control, to ensure accurate operation of the instruments. The staff are well qualified to perform routine instrument maintenance, plus minor repairs. In addition, they assist investigators with data analysis and storage, as well as the collection of information for facility usage reporting, billing users for services provided, plus the purchase of supplies, as required. They provide appropriate training for investigators, students and technical staff in the operation of the flow cytometers, use of their software and analysis of data.

Shannon Opiela, Ph.D., Research Associate

Dr. Opiela received her Ph.D. in immunology from the University of Miami Miller School of Medicine in 2008. She has had six years of successfully designing and interpreting flow cytometry-based experiments in the laboratory of her mentor, Dr. Becky Adkins, which have led to several top-tier publications. She has further expanded her knowledgebase by becoming proficient with an array of flow cytometry applications that span the MAC/PC platforms. In addition to her technical knowledge, Dr. Opiela has a comprehensive understanding of biological systems; specifically, immune cell function. This has given her a broad understanding of cell surface molecules as well as intracellular molecules which can be used to both phenotypically and functionally characterize cell subsets; granting the FCCF user maximum flexibility.

Jay Enten, B.S., Research Associate

Mr. Enten holds a Bachelors of Science degree in biology from Villanova University. His professional career started immediately after graduation in the quality control department of a small startup company in Maryland. Mr. Enten took his quality control knowledge to Coulter Corporation in 1984 where his experience spanned 22 years. During this tenure he was a vital member of teams that developed flow cytometry instruments, software, and applications while providing product and technical support to customers. Upon leaving Beckman Coulter in 2005, he started his own consulting company that provided services to clients in need of technical support and applications development in flow cytometry.

Patricia Guevara, B.S., M.A., Research Associate

Mrs. Guevara received her Bachelors of Science degree in Microbiology and Immunology and a Master of Arts in Liberal Studies from the University of Miami. Shortly after completing her bachelors, she began her scientific career in the laboratory of Dr. Becky Adkins at the University of Miami, Miller School of Medicine. She became proficient in designing, implementing and troubleshooting experiments using biological, molecular and flow cytometry methods. During her 13 year tenure in the Adkins laboratory, she has gained extensive knowledge in flow cytometry applications that were essential to her research in neonatal immunology. Her experience encompasses use and knowledge of the analyzers, sorters and the analysis software, FlowJo.

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