Sylvester Comprehensive Cancer Center

Flow Cytometry Services

Fluorescence Flow Cytometry Analysis and Fluorescent Activated Cell Sorting

The FCSR provides sophisticated analytical and preparative flow cytometers to facilitate a wide range of experiments in the cell biology of both normal and cancer cells. The facility has the capability to provide simultaneous analysis of up to 19 different fluorescent parameters and two light-scatter parameters (forward and side scatter), for both cell surface and cytoplasmic antigens in complex cell mixtures. A variety of experimental analyses are commonly performed in the facility. These include:

  • Complex 19 fluorescence parameter immuno-phenotyping
  • DNA content, ploidy and cell cycle analysis
  • Up to 17 fluorescence parameter cell sorting and single-cell flow cloning
  • High speed (10,000 – 50,000+ cells per second) and efficiency (95%+ purity) cell sorting
  • Assessment of apoptosis
  • Molecular cytogenetics
  • Cell function studies
  • Micro-biological applications
  • Multiplexed and micro-particle-based analysis

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