Sylvester Comprehensive Cancer Center

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Oncogenomics Core Facility

The Oncogenomics Core Facility (OCF) is a state-of-the-art open-core facility located in the in the Biomedical Research Building, which houses John P. Hussman Institute for Human Genomics, at the University of Miami. The mission of the OCF is to provide the members of the Sylvester Comprehensive Cancer Center with state-of-the-art technical services for the study of the genomics and epigenetics of cancer. The OCF provides investigators access to a wide range of technologies and services for the molecular analysis of DNA and RNA in addition to providing technical expertise for project design and trouble-shooting. The OCF employs four full-time technicians overseen by a Faculty-level Director. Services span RNA/DNA extraction and QC, droplet digital-PCR, PCR-free gene expression assays, single-cell sample prep and gene expression analysis, miRNA and CNV assays, microarrays and rapid run next generation sequencing for RNA-Seq ChIP-Seq and other NGS workflows. OCF facilities, instrumentation and bench space, are divided into sample processing (pre-PCR) and analytical laboratories (post-PCR) in two separate open-plan laboratories. All equipment is maintained under rolling service contracts with regular preventative maintenance.

OCF instrumentation includes:

  • Raindance Technologies Raindrop droplet digital ddPCR system
  • BioRad QX200 droplet digital ddPCR system
  • Fluidigm C1 single-cell auto-prep station and Biomark HD nanofluidic qPCR system
  • NanoString PrepStation and nCounter analysis systems
  • Illumina NextSeq 500 sequencer (NGS)
  • Illumina iScan microarray scanner
  • Two Roche Lightcycler LC480 real-time PCR machines fed by a PE/Caliper Twister II autoloader and M├ęcour refrigerated sample storage system
  • Bio-Rad C1000 deep-well and Eppendorf Mastercycler Pro-S PCR systems
  • Thermo Scientific Nanodrop, LifeTechnologies Qubit and Agilent Bioanalyzer 2100 systems
  • Eppendorf epMotion 5075 and Beckman Coulter Biomek NXP automation systems

Contact Us





Mailing Address

1550 N.W. 10th Avenue, FOX 100 (M877)
Miami, FL 33101

Physical Address

Biomedical Research Building (BRB) 542C
1501 N.W. 10th Avenue
Miami, FL 33136

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