Capillary sequencing is performed on an Applied Biosystems Prism 3130XL DNA Analyzer. We use BigDye 3.1 and ET Terminator chemistry. Standard primers are provided by the facility at no extra charge (see FAQ). Custom primers should be provided by the user. The turnaround time for receiving sequencing results depends on the volume of sequencing requests to be processed. The standard turnaround, during a 5 day work week, is 24 to 48 hrs. after receipt of a DNA template. When the number of samples for sequencing exceeds the daily capacity of the lab, the turnaround time increases, generally to no more than 48 hr. More information is available in this Capillary Sequencing FAQ document.
Applied Biosystems 3130xl Genetic Analyzer: Enables a wide variety of sequencing and fragment analysis applications, including microsatellite analysis, AFLP, loss of heterozygosity (LOH), SNP validation, and SNP screening, as well as de novo sequencing and resequencing for mutation analysis. Key applications include de novo sequencing, mutation discovery, target verification, and fragment analysis.