Sylvester Comprehensive Cancer Center

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Second Generation Sequencing

(Also known as Next-Generation Sequencing, Deep Sequencing, Ultra-High Throughput Sequencing) Through our partnership with the Hussman Institute for Human Genomics-Center For Genome Technology we have access to Illumina GA IIx and HiSeq2000 second generation sequencer. The GAIIx provides massively parallel sequencing of random fragments. Each of the seven lanes available on a flow cell typically generate 25 to 60 million separate reads with a total throughput of 1.5 to 5.5 gigabases of sequence data per lane per run. Read lengths of 36, 52, and 76 base pairs are possible which, when combined with the ability to perform paired-end sequencing, yield up to 152 base pair reads from each fragment. Four to twelve samples may be multiplexed in a single lane. Appropriate applications include ChIP-seq, RNA-seq, microRNA-seq, targeted sequencing (sequence capture), de-novo sequencing of small genomes, and resequencing of any genome when a reference genome is available. Run times vary depending on read length and are in the three to nine day range. Please inquire about sequencing on theHiSeq2000 system.


Illumina Genome Analyzer (GA) IIx

Beckman Coulter Biomek NXp Robotics Platform: This robotics platform is configured for high throughput liquid handling and is used in standardized RNA and DNA extraction procedures from cells, FFPE and fresh frozen tissue. It is also used for molecular diagnostics assays for research. The Biomek is located in an adjacent laboratory, separate from the analysis facility to maintain sample integrity and prevent contamination.

Please contact us for a quote on your project.

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