Sylvester Comprehensive Cancer Center

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ES Cell Culture

This aspect of the procedure will be responsible for the culture and gene targeting of mouse ES cells required for the production of gene targeted mice.

Services will include:

  • Culture of ES cells with LIF and feeder cells as required
  • Electroporation of ES cells with the targeting vector
  • Drug selection of transfected ES cells and isolation of drug resistant clones
  • Expansion of ES clones for screening and cryopreservation
  • Re-culture of cryopreserved clones for microinjection into blastocysts

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