Sylvester Comprehensive Cancer Center

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Molecular Biology

This aspect, currently under construction will help researchers prepare the targeting vector for the generation of gene knockout and knockin mice. It will offer the following services:

  • Analysis of genomic DNA sequence
  • Design of targeting vector(s)
  • Identification of BAC clones corresponding to the gene of interest
  • Sequence annotation, generation, and analysis of the genomic insert
  • Preparation of molecular probes for genotyping and prediction of the targeting insertion site
  • Southern analysis of the targeting construct and genomic DNA
  • Purification of the targeting constructs for electroporation into ES cells

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